TY - JOUR AU - Alcántar-Vázquez, Juan Pablo AU - Dumas, Sylvie AU - Peña, Renato AU - Ibarra, Ana M PY - 2016/06/15 Y2 - 2024/03/28 TI - Effects of cold-shock triploidy induction treatment on cell cleavage, hatching rate, and survival of triploid larvae of spotted sand bass (Paralabrax maculatofasciatus) JF - Ciencias Marinas JA - Cienc. Mar. VL - 42 IS - 2 SE - Research Article DO - 10.7773/cm.v42i2.2581 UR - https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2581 SP - 111-122 AB - <p>Three experiments assessed the effects of cold-shock induced triploidy on (1) the incidence of abnormal cell cleavage during embryogenesis, (2) hatching success and the proportion of live yolk-sac larvae in normally cleaved versus abnormally cleaved embryos, and (3) survival of triploid larvae of spotted sand bass (<em>Paralabrax maculatofasciatus</em>). All spawns were obtained by hormonal induction (LHRHa). Triploidy was induced by cold shock shortly after fertilization. In experiment 1, the percentage of abnormal cell cleavage was significantly higher (<em>P</em> &lt; 0.05) in the cold-shocked group than in the control group, while the hatching rate and the proportion of live yolk-sac larvae were significantly lower (<em>P</em> &lt; 0.05). In experiment 2, when normally and abnormally cleaved embryos from the control and cold-shocked groups were incubated separately, normal cleaved embryos in the cold-shocked group showed a significantly higher hatching rate and proportion of live yolk-sac larvae than abnormally cleaved embryos in the same group. No difference was observed in the control group. In experiment 3, a significant decrease (<em>P</em> &lt; 0.05) in the triploidy count was observed throughout the larval period. Final survival was drastically lower for triploid larvae compared to cold-shocked diploids and untreated eggs. Low triploidy percentages at the end of the larval period would seriously hinder the development of triploid sand bass culture.</p> ER -